METHOD FOR DETECTING SINGLE-STRANDED RNA VIRUS

A method for detecting a single-stranded RNA virus in a sample includes: bringing a primer set into contact with a sample to perform a RT-LAMP reaction, wherein the primer set is designed based on a nucleotide sequence of a target RNA and a nucleotide sequence of a nucleic acid complementary to the...

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Bibliographische Detailangaben
Hauptverfasser:	SEMBA, Syouhei, MICHIYUKI, Satoru, YOKONO, Kota, YAMAMOTO, Machiko
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for detecting a single-stranded RNA virus in a sample includes: bringing a primer set into contact with a sample to perform a RT-LAMP reaction, wherein the primer set is designed based on a nucleotide sequence of a target RNA and a nucleotide sequence of a nucleic acid complementary to the target RNA, and includes following (i) to (v): (i) an FIP primer; (ii) a BIP primer; (iii) an F3 primer, which is an outer primer; (iv) a B3 primer, which is an outer primer; and (v) one or more additional outer primers, each additional outer primer having the same nucleotide sequence as an arbitrary region present on a 5′ terminal side from the region B3 or the region F3 in the nucleic acid complementary to the target RNA. According to the present invention, a single-stranded RNA virus can be detected with high sensitivity.