HIGH THROUGHPUT SINGLE-CHAMBER PROGRAMMABLE NUCLEASE ASSAY

Disclosed herein are systems and methods for providing a high-throughput DETECTR assay in a single chamber. The single chamber may be one well of a microplate, and multiple assays may be conducted in a staggered fashion in separate chambers. The methods described herein implement a process including...

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Bibliographische Detailangaben
Hauptverfasser:	BROUGHTON, James Paul, HIRSCHI, Alexander, PATNO, Timothy James, MIAO, Xin, FASCHING, Clare Louise, TONDNEVIS, Farzaneh, ANSALONI, Sara, VERMA, Deepika, ADDISON, II, Wesley Wendell, CHEN, Janice Sha, MEI, Raymond Weibang, CHING, Jesus, ALPAY, Nazmiye Emel
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed herein are systems and methods for providing a high-throughput DETECTR assay in a single chamber. The single chamber may be one well of a microplate, and multiple assays may be conducted in a staggered fashion in separate chambers. The methods described herein implement a process including lysing a sample, isolating nucleic acid molecules, eluting the nucleic acid molecules, amplifying the nucleic acid molecules, and detecting a presence of a target nucleic acid.