A METHOD OF SINGLE-STRANDED RNA PURIFICATION

A method of single-stranded RNA purification comprising the stepsapplying a sample containing single-stranded RNA to a solid phase bearing dominantly or exclusively primary amino groups on its surface, at a pH value sufficient to bind at least predominantly the single-stranded RNA,eluting the single...

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Bibliographische Detailangaben
Hauptverfasser: PERSIC, Spela, CERNIGOJ, Urh, DOLENC, Darko, GAGNON, Peter S
Format: Patent
Sprache:eng
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Zusammenfassung:A method of single-stranded RNA purification comprising the stepsapplying a sample containing single-stranded RNA to a solid phase bearing dominantly or exclusively primary amino groups on its surface, at a pH value sufficient to bind at least predominantly the single-stranded RNA,eluting the single-stranded RNA from the surface of the solid phase by exposing the surface of the solid phase to increasing pH.