A METHOD OF SINGLE-STRANDED RNA PURIFICATION

A method of single-stranded RNA purification comprising the steps:applying a sample containing single-stranded RNA to a solid phase dominantly or exclusively bearing both primary and secondary amino groups on its surface under conditions sufficient to bind at least predominantly the single-stranded...

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Bibliographische Detailangaben
Hauptverfasser:	PERSIC, Spela, CERNIGOJ, Urh, DOLENC, Darko, GAGNON, Peter S
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of single-stranded RNA purification comprising the steps:applying a sample containing single-stranded RNA to a solid phase dominantly or exclusively bearing both primary and secondary amino groups on its surface under conditions sufficient to bind at least predominantly the single-stranded RNA,eluting the single-stranded RNA adsorbed on the surface of the solid phase from the solid surface by means of an elution buffer having a higher concentration of comprising soluble pyrophosphate anions than needed for desorbing double-stranded RNA.