Display Substrate and Manufacturing Method Thereof, and Display Apparatus

The present disclosure provides a display substrate, a manufacturing method thereof and a display apparatus. The display substrate includes a base substrate, an active structure layer disposed on the base substrate and a source-drain structure layer disposed on the active structure layer. The active structure layer includes a first active layer and a second active layer. A material of the first active layer includes low-temperature polysilicon and a material of the second active layer includes oxide semiconductors. The source-drain structure layer includes a first source-drain electrode and a second source-drain electrode, wherein the first source-drain electrode overlaps with a first side surface of the first active layer through a first via hole and the second source-drain electrode overlaps with a second side surface of the second active layer through a second via hole.