NOVEL QUENCHER AND REPORTER DYE COMBINATIONS

Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: EVANS, Brian, BENSON, Scott C, CHANG, Chu-An, MULLAH, Khairuzzaman Bashar, YAN, Xiongwei
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.