METHODS AND SYSTEMS FOR CLASSIFYING FLOW CYOMETER DATA

Methods of classifying flow cytometer data are provided. Methods of interest include receiving a first gate and flow cytometer data, expanding the first gate to generate a second gate, and determining sets of flow cytometer data encompassed by each of the first gate and the second gate to classify t...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wolf, Christopher J, Roberts, David Alexander, Owsley, Keegan
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Methods of classifying flow cytometer data are provided. Methods of interest include receiving a first gate and flow cytometer data, expanding the first gate to generate a second gate, and determining sets of flow cytometer data encompassed by each of the first gate and the second gate to classify the flow cytometer data. In embodiments, methods also involve recording a subset of the classified flow cytometer data and optionally adjusting the first and/or second gates based on the recorded data. In some cases, the subject methods include sorting particles associated with the classified flow cytometer data based on the first and second gates. Systems and computer-readable storage media for practicing the invention are also provided.