NUCLEIC ACID MASS SPECTRUM NUMERICAL PROCESSING METHOD
A numerical processing method for a nucleic acid mass spectrum, including: step S1, recalibrating a single mass spectrum, for each detection point of a sample, obtaining a plurality of mass spectra corresponding to different positions of the detection point, each mass spectrum being recalibrated by...
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Zusammenfassung: | A numerical processing method for a nucleic acid mass spectrum, including: step S1, recalibrating a single mass spectrum, for each detection point of a sample, obtaining a plurality of mass spectra corresponding to different positions of the detection point, each mass spectrum being recalibrated by using anchor peaks with an expected mass-to-charge ratio; step S2, synthesizing the mass spectra, where the mass spectra corresponding to the different positions of the detection point are synthesized into a unitary mass spectrum of the detection point; step S3: performing wavelet filtering on the unitary mass spectrum to eliminate high-frequency noise and a baseline through a wavelet-based digital filter; and step S4: extracting a peak feature value, performing peak fitting to obtain a fitted curve of the unitary mass spectrum, and obtaining a peak height, a peak width, a peak area, a mass offset, and a signal-noise ratio based on the fitted curve. |
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