ESTIMATING A QUANTITY OF MOLECULES IN A SAMPLE

A synthetic molecule can be added to a sample at a specified concentration to accurately and/or precisely quantify target molecules included in the sample. The synthetic molecule can include a number of nucleotides. Some of the regions of the synthetic molecule can include sequences that correspond...

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Bibliographische Detailangaben
Hauptverfasser: Ishoey, Thomas, Clement, Brian, Sanders, Jonathan, Callahan, Heather
Format: Patent
Sprache:eng
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Zusammenfassung:A synthetic molecule can be added to a sample at a specified concentration to accurately and/or precisely quantify target molecules included in the sample. The synthetic molecule can include a number of nucleotides. Some of the regions of the synthetic molecule can include sequences that correspond to primers used in an amplification process and other regions of the synthetic molecule can include sequences that are machine-generated. In implementations, an initial number of target molecules included in the sample can be determined based on a number of the target molecules included in an amplification product in relation to the number of synthetic molecules added to the sample.