DROPLET MICROFLUIDIC PLATFORM FOR THE ENHANCED DNA TRANSFER BETWEEN MICROBIAL SPECIES

In an embodiment, the present disclosure pertains to a microfluidic platform composed of a droplet generator having an entry point for donor particles and target particles, a first droplet incubation chamber in fluid communication with the droplet generator, a droplet detection functionality to allo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	Adams, Bryn L, Wippold, Jose, Han, Arum
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	In an embodiment, the present disclosure pertains to a microfluidic platform composed of a droplet generator having an entry point for donor particles and target particles, a first droplet incubation chamber in fluid communication with the droplet generator, a droplet detection functionality to allow for analysis of the inner content of droplets, and a droplet sorting functionality to allow for the separation of droplets based on the analysis of the inner content of droplets. In another embodiment, the present disclosure pertains to a method for cell-to-cell DNA, RNA, or other genetic material transfer through use of a water-in-oil emulsion microdroplet-based microfluidic platform for automation and high throughput identification or screening of genetic transfer outcomes utilizing the microfluidic platforms as disclosed herein.