METHOD FOR IDENTIFYING M1 GENERATION PLANT MUTANTS RESULTING FROM PHYSICAL AND CHEMICAL MUTAGENESIS AND FOR ACQUIRING MUTANT, IDENTIFICATION OF GENOTYPING PRIMER FOR ORYZA SATIVA MUTATION, MUTANT GENE, AND USE THEREOF
The present disclosure discloses a method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis, a method for acquiring the plant mutant, a mutant gene, and use thereof. The method for identifying a M1 generation plant mutant resulting from physical and chemic...
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| creator | Tang, Li Li, Yaokui Mao, Bigang Peng, Yan Yu, Lixia Li, Wenjian Zhang, Dan Shao, Ye Bai, Lianyang Yuan, Longping Du, Yan Hu, Yuanyi Zhao, Bingran |
| description | The present disclosure discloses a method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis, a method for acquiring the plant mutant, a mutant gene, and use thereof. The method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis includes: mutagenizing a plant to obtain an M1 generation plant mutant, extracting a mixed pool of DNA from the obtained M1 generation plant mutant, subjecting the mixed pool of DNA to high-depth targeted sequencing for a target gene region, and aligning a sequencing result with the target gene region to identify whether there are target single nucleotide polymorphisms (SNPs) and/or Indel. The method of the present disclosure has high efficiency and high accuracy, involves simple operations, and is of progressive significance for the identification and acquisition of innovative germplasms. |
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The method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis includes: mutagenizing a plant to obtain an M1 generation plant mutant, extracting a mixed pool of DNA from the obtained M1 generation plant mutant, subjecting the mixed pool of DNA to high-depth targeted sequencing for a target gene region, and aligning a sequencing result with the target gene region to identify whether there are target single nucleotide polymorphisms (SNPs) and/or Indel. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD FOR IDENTIFYING M1 GENERATION PLANT MUTANTS RESULTING FROM PHYSICAL AND CHEMICAL MUTAGENESIS AND FOR ACQUIRING MUTANT, IDENTIFICATION OF GENOTYPING PRIMER FOR ORYZA SATIVA MUTATION, MUTANT GENE, AND USE THEREOF |
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