METHOD FOR IDENTIFYING M1 GENERATION PLANT MUTANTS RESULTING FROM PHYSICAL AND CHEMICAL MUTAGENESIS AND FOR ACQUIRING MUTANT, IDENTIFICATION OF GENOTYPING PRIMER FOR ORYZA SATIVA MUTATION, MUTANT GENE, AND USE THEREOF

The present disclosure discloses a method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis, a method for acquiring the plant mutant, a mutant gene, and use thereof. The method for identifying a M1 generation plant mutant resulting from physical and chemical mutagenesis includes: mutagenizing a plant to obtain an M1 generation plant mutant, extracting a mixed pool of DNA from the obtained M1 generation plant mutant, subjecting the mixed pool of DNA to high-depth targeted sequencing for a target gene region, and aligning a sequencing result with the target gene region to identify whether there are target single nucleotide polymorphisms (SNPs) and/or Indel. The method of the present disclosure has high efficiency and high accuracy, involves simple operations, and is of progressive significance for the identification and acquisition of innovative germplasms.