METHODS FOR TRANSFORMATION OF FUNGAL SPORES

The present invention provides to a method for the introducing polynucleotide molecules into fungal spores comprising exposing a mixture of fungal spores and magnetic nanoparticles carrying the polynucleotide to a magnet and/or a magnetic field. The present invention also provides a method for trans...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	Stuiver, Maarten Hendrik, Hoff, Birgit, Jacob, Stefan, Thines, Eckhard, Siepe, Isabella, Yemelin, Alexander, Mentzel, Tobias, Becker, Michael, Schachtschabel, Doreen
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides to a method for the introducing polynucleotide molecules into fungal spores comprising exposing a mixture of fungal spores and magnetic nanoparticles carrying the polynucleotide to a magnet and/or a magnetic field. The present invention also provides a method for transformation a fungus comprising the steps of the method steps disclosed herein and allowing the integration of said polynucleotide molecules into the genome of said spores, thereby transforming the spore. The present invention also provides a system for delivery of nucleic acids to fungal spores comprising magnetic nanoparticles loaded with nucleic acids, a kit for transformation of fungal spores with a polynucleotide comprising MNPs loaded with the polynucleotide and fungal spores as well as a composition comprising MNPs loaded with nucleic acid molecules and fungal spores.