CELL CULTURE METHOD AND APPLICATION THEREOF BASED ON HIGH-DENSITY AND CONTINUOUS INOCULATION

The present invention discloses a cell culture method based on high-density and continuous inoculation and use thereof. The method comprises the following steps: (1) providing a cell culture, and performing resuscitation, shake flask amplification culture and rocking reaction bag amplification culture on the cell culture; (2) transferring the resuscitated and amplified cells into a last-stage cell amplification tank for continuous amplification culture; (3) inoculating the cells in the last-stage cell amplification tank into a culture fermentation tank in a high-density and continuous inoculation mode for fermentation culture; and (4) harvesting a target product. The cell culture method based on high-density and continuous inoculation provided herein can reduce the average cell passage time of each batch and improve the production efficiency of cells and expression products thereof.