Methods for cryopreservation of sub-millimeter and millimeter scale biological materials

Methods for cryopreservation of biological samples are provided. The biological samples are sub-millimeter or millimeter scale biological materials. The biological samples are embryos, such as Drosophila embryos. Methods for cryopreservation of Drosophila embryos using cryomesh are provided. The Dro...

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Hauptverfasser: Zhan, Li, Li, Mingang, Hays, Thomas, Bischof, John
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creator Zhan, Li
Li, Mingang
Hays, Thomas
Bischof, John
description Methods for cryopreservation of biological samples are provided. The biological samples are sub-millimeter or millimeter scale biological materials. The biological samples are embryos, such as Drosophila embryos. Methods for cryopreservation of Drosophila embryos using cryomesh are provided. The Drosophila embryos are collected, staged and treated to optimize the cryopreservation outcomes upon rewarming. Methods disclosed are efficient for maintaining stocks of Drosophila wild type and mutant strains. Methods are also disclosed for cryopreservation of other terrestrial organism embryos and/or aquatic organism embryos.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
CARE OF BIRDS, FISHES, INSECTS
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW BREEDS OF ANIMALS
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
TRAPPING
title Methods for cryopreservation of sub-millimeter and millimeter scale biological materials
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