Methods for cryopreservation of sub-millimeter and millimeter scale biological materials

Methods for cryopreservation of biological samples are provided. The biological samples are sub-millimeter or millimeter scale biological materials. The biological samples are embryos, such as Drosophila embryos. Methods for cryopreservation of Drosophila embryos using cryomesh are provided. The Dro...

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Bibliographische Detailangaben
Hauptverfasser:	Zhan, Li, Li, Mingang, Hays, Thomas, Bischof, John
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CARE OF BIRDS, FISHES, INSECTS FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW BREEDS OF ANIMALS PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR TRAPPING
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Beschreibung
Zusammenfassung:	Methods for cryopreservation of biological samples are provided. The biological samples are sub-millimeter or millimeter scale biological materials. The biological samples are embryos, such as Drosophila embryos. Methods for cryopreservation of Drosophila embryos using cryomesh are provided. The Drosophila embryos are collected, staged and treated to optimize the cryopreservation outcomes upon rewarming. Methods disclosed are efficient for maintaining stocks of Drosophila wild type and mutant strains. Methods are also disclosed for cryopreservation of other terrestrial organism embryos and/or aquatic organism embryos.