Droplet Sorting Decision Modules, Systems and Methods of Use Thereof

Droplet sorting modules for sorting droplets of a flow stream are described. Droplet sorting modules according to certain embodiments include a plurality of droplet sort decision units and a processor with memory operably coupled to the processor, where the memory includes instructions stored thereon, which when executed by the processor, cause the processor to determine an optimal droplet sort decision unit from the plurality of sort decision units for sorting each droplet of the flow stream. Particle (e.g., cells) sorting systems and methods for sorting droplets of a flow stream are also described. Kits having one or more of the subject droplet sorting modules are also provided.