METHOD FOR INDEX SORTING UNIQUE PHENOTYPES AND SYSTEMS FOR SAME

Aspects of the present disclosure include methods for flow cytometrically sorting a sample with particles, such as cells, based on order of identification. Methods according to certain embodiments include introducing the sample into a flow cytometer; flowing the introduced sample in a flow stream; i...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wei, Lingjie, Mage, Peter, Roberts, David Alexander, Owsley, Keegan
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Aspects of the present disclosure include methods for flow cytometrically sorting a sample with particles, such as cells, based on order of identification. Methods according to certain embodiments include introducing the sample into a flow cytometer; flowing the introduced sample in a flow stream; irradiating the sample in the flow stream with a light source; detecting light from cells in the sample flowing in the flow stream; identifying phenotypes of cells in the sample flowing in the flow stream based on one or more data signals generated from the detected light; and dynamically sorting into partitions cells of the sample that have a phenotype of a collection of predetermined phenotypes based on order of identification. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.