METHOD FOR CONTINUOUSLY COLLECTING FLUORESCENCE DATA OF MICROFLUIDIC CHIP

A method for continuously collecting fluorescence data of a microfluidic chip is provided. In the method, an optical path system emits a light perpendicular to the microfluidic chip, such that a center of a light spot formed by the optical path system on the microfluidic chip is located on a circle...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG, Guanbin, LI, Ruoran, SONG, Chicheng, TAO, Yong, ZHOU, Hongcheng, HUANG, Yong
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	A method for continuously collecting fluorescence data of a microfluidic chip is provided. In the method, an optical path system emits a light perpendicular to the microfluidic chip, such that a center of a light spot formed by the optical path system on the microfluidic chip is located on a circle formed by centers of all reaction cells. The microfluidic chip is rotated around a center of the circle formed by centers of all the reaction cells. Fluorescence signal values are collected by using the optical path system along a rotation direction of the microfluidic chip. The collected fluorescence signal values are processed to obtain effective fluorescence data of all the reaction cells.