Detection of Optimal Recombinants Using Fluorescent Protein Fusions

A detection of optimal genetic recombinants used to prepare target proteins, with assessment of their target-specific "upstream" productivity, genetic stability and means to optimize target protein "downstream" purification using customizable fluorescent tags. A scarless removabl...

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Hauptverfasser:	Fotheringham, Ian, McElroy, David, Erskine, Harveen, Magneschi, Leonardo, Baxter, Scott, McColm, Stephen, Kjeldsen, Annemette, Serrano-Amatriain, Cristina, Kay, Jack Eric
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	A detection of optimal genetic recombinants used to prepare target proteins, with assessment of their target-specific "upstream" productivity, genetic stability and means to optimize target protein "downstream" purification using customizable fluorescent tags. A scarless removable protein fusion makes it possible to identify recombinants of Pichia pastoris with optimal performance in heterologous protein production.