SCALABLE TAGGING OF ENDOGENOUS GENES BY HOMOLOGY-INDEPENDENT INTRON TARGETING

Provided herein are nucleic acid compositions and methods of their use for integrating reporter proteins, such as split fluorophore protein fragments, into intronic genomic regions. The integrated sequence is flanked by a splice acceptor site and a splice donor site such that the reporter protein se...

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Hauptverfasser:	SHALEM, Ophir H, SANSBURY, Stephanie E, SEREBRENIK, Yevgeniy V
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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