SCALABLE TAGGING OF ENDOGENOUS GENES BY HOMOLOGY-INDEPENDENT INTRON TARGETING

SCALABLE TAGGING OF ENDOGENOUS GENES BY HOMOLOGY-INDEPENDENT INTRON TARGETING

Provided herein are nucleic acid compositions and methods of their use for integrating reporter proteins, such as split fluorophore protein fragments, into intronic genomic regions. The integrated sequence is flanked by a splice acceptor site and a splice donor site such that the reporter protein se...

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Bibliographische Detailangaben
Hauptverfasser: SHALEM, Ophir H, SANSBURY, Stephanie E, SEREBRENIK, Yevgeniy V
Format: Patent
Sprache:eng
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
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Beschreibung
Zusammenfassung:Provided herein are nucleic acid compositions and methods of their use for integrating reporter proteins, such as split fluorophore protein fragments, into intronic genomic regions. The integrated sequence is flanked by a splice acceptor site and a splice donor site such that the reporter protein sequence is incorporated into the mature mRNA expressed from the target gene.