METHODS AND APPARATUS FOR SAMPLE ANALYSIS USING LATERAL FLOW

Methods and assays for performing a lateral flow test are disclosed. A sample is applied to a receiving portion of a lateral flow device such that the sample flows to at least a first test zone and a second test zone. First and second signals levels at the first and second test zones over an assay period are monitored. If a first analyte of interest is present in the sample, the first analyte is labelled and the presence of labelled first analyte in the sample causes one of the first and second signals levels to increase during the assay period. A change between the first and second signal levels over a period of time during the assay period is monitored. The sample can be incubated prior to application to the receiving portion to provide homogeneity to labelling and therefore a substantially linear increase of the signal level at one of the test zones and a substantially constant signal level at the other one of the test zones.