Method for Identifying Mastitis-Causing Microbes

Provided herein is a method for identifying a mastitis-causing microbe in a subject. A milk sample is centrifuged to form a microbial pellet, total nucleic acids are extracted from the pellet and a microarray analysis of extracted DNA from which the mastitis-causing microbe is identified from DNA hy...

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Bibliographische Detailangaben
Hauptverfasser: May, Melissa R, Eggers, Frederick H, Hogan, Michael E
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Provided herein is a method for identifying a mastitis-causing microbe in a subject. A milk sample is centrifuged to form a microbial pellet, total nucleic acids are extracted from the pellet and a microarray analysis of extracted DNA from which the mastitis-causing microbe is identified from DNA hybridization to mastitis-causing microbe species-specific gene probes. Also provided is a method for diagnosing a bovine mastitis infection in a dairy cow after identifying the bovine mastitis-causing microbe in a raw milk sample from the dairy cow.