PROGRAMMABLE MULTIPLE-POINT ILLUMINATOR, CONFOCAL FILTER, CONFOCAL MICROSCOPE AND METHOD TO OPERATE SAID CONFOCAL MICROSCOPE

A programmable multiple-point illuminator for an optical microscope (M) including a light source and a spatial light modulator (SLM) to modulate a light beam from the light source. The modulated light beam scans across a sample placed under the microscope objective, the sample being provided with fl...

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Bibliographische Detailangaben
Hauptverfasser:	MARTÍN BADOSA,, Estela, TREPTOW, Dorian, BOLA SAMPOL, Raúl, MONTES USATEGUI, Mario
Format:	Patent
Sprache:	eng
Schlagworte:	CINEMATOGRAPHY DEVICES OR ARRANGEMENTS, THE OPTICAL OPERATION OF WHICH ISMODIFIED BY CHANGING THE OPTICAL PROPERTIES OF THE MEDIUM OF THEDEVICES OR ARRANGEMENTS FOR THE CONTROL OF THE INTENSITY,COLOUR, PHASE, POLARISATION OR DIRECTION OF LIGHT, e.g.SWITCHING, GATING, MODULATING OR DEMODULATING ELECTROGRAPHY FREQUENCY-CHANGING HOLOGRAPHIC PROCESSES OR APPARATUS HOLOGRAPHY NON-LINEAR OPTICS OPTICAL ANALOGUE/DIGITAL CONVERTERS OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS OPTICAL LOGIC ELEMENTS OPTICS PHOTOGRAPHY PHYSICS TECHNIQUES OR PROCEDURES FOR THE OPERATION THEREOF
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Beschreibung
Zusammenfassung:	A programmable multiple-point illuminator for an optical microscope (M) including a light source and a spatial light modulator (SLM) to modulate a light beam from the light source. The modulated light beam scans across a sample placed under the microscope objective, the sample being provided with fluorophores. The SLM includes a first acousto-optic deflector and a second acousto-optic deflector, the first acousto-optic deflector having a first modulation plane and the second acousto-optic deflector having a second modulation plane, said two acousto-optic deflectors being arranged in cascade to provide respective deflection in different directions, whereby the SLM is enabled to scan in two dimensions across the sample. The SLM further a telescope relay to conjugate the first modulation plane with the second modulation plane. The illuminator also has an arbitrary waveform generator that is configured to synthesize holograms, and is arranged to simultaneously inject a first such hologram into the first acousto-optic deflector and a second such hologram into the second acousto-optic deflector, in order for the SLM to modulate the light beam in response to said holograms.