METHOD FOR EFFICIENTLY RECOVERING AND PURIFYING ACTIVE CRM197 FROM INSOLUBLE CRM197 PROTEIN EXPRESSED IN INCLUSION BODY

METHOD FOR EFFICIENTLY RECOVERING AND PURIFYING ACTIVE CRM197 FROM INSOLUBLE CRM197 PROTEIN EXPRESSED IN INCLUSION BODY

Disclosed is a method for expressing and purifying insoluble proteins of CRM197. The method includes: culturing a transformant transformed with an expression vector containing a gene encoding CRM197 protein; obtaining a culture of the transformant and disrupting the cell to recover an inclusion body...

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Bibliographische Detailangaben
Hauptverfasser: LEE, Hyeon Cheol, SEO, Hyoung Jong, KOO, Bong Seong, PARK, Ah Reum, JANG, Seung Won, KIM, Jin Sook
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:Disclosed is a method for expressing and purifying insoluble proteins of CRM197. The method includes: culturing a transformant transformed with an expression vector containing a gene encoding CRM197 protein; obtaining a culture of the transformant and disrupting the cell to recover an inclusion body; solubilizing the inclusion body; treating the solubilized inclusion body with a refolding reaction mixture and performing a refolding process; and purifying the refolded CRM197 protein by chromatography.