Method of enhancing isothermal amplification sensitivity of nucleic acid and reagents thereof

Disclosed herein is a method of enhancing RAA isothermal amplification sensitivity using magnetic beads and applications in nucleic acid analysis and detection and medical diagnosis, belonging to the biomedical engineering field, comprising: (1) material selection of magnetic beads, (2) optimization of diameter of magnetic beads, (3) optimization of mixing time of magnetic beads, and (4) sensitivity detection method of magnetic beads for RAA isothermal detection, wherein the material of magnetic bead is steel bead, the diameter of the magnetic bead is 1.5 mm, the mixing time of the magnetic bead is 30 s, and the detection method of isothermal RAA results includes agarose gel and fluorescence detection. Under this condition, the sensitivity of magnetic beads for RAA isothermal amplification is increased by 10 times compared to that without adding magnetic beads, and increased by 100 times compared to that of other types of fluorescence detectors. The method disclosed herein can significantly enhance the sensitivity of RAA isothermal amplification, and have a wide application in rapid detection of nucleic acids and clinical diagnosis.