NEOANTIGEN PREPARATIONS AND USES THEREOF

Improved and more robust vaccine preparations (such as cancer vaccines), and methods of treating a subject having a neoplastic cell growth, such as a cancer or tumor, are presented. A neoantigen component having one or more identified neoantigens that are overexpressed in a tumor cell or cancer cell is provided. The overexpressed neoantigens are in some examples MEW Class I neoantigens. Personalized neoantigen preparations for a subject to be used with the vaccine are also provided. Methods of screening a biological sample from a subject having a cancer or tumor to identify specific neoantigens overexpressed after treatment with the tissue vaccine are presented. A panel of neoantigens characteristic of a specific cancer or tumor is also provided. The preparations, methods and panels are provided for subjects capable of or at risk of developing a tumor, cancer, or related disease. Methods and preparations for treating malignant, pre-malignant, and at-risk subjects are also presented.