APPARATUS AND METHOD OF AIR-SUSPENDED BIOFABRICATION OF TISSUE-ENGINEERED ORGAN CONSTRUCTS AND CONGLOMERATES OF SPHEROIDS, CELLS AND OTHER BIOLOGICAL OBJECTS BY USING MAGNETIC FIELD

The present invention generally relates to biofabrication technology and, more particularly, to systems and methods for manufacturing three-dimensional constructs made of various materials using scaffold-free, nozzle-free and label-free magnetic levitation in non-toxic paramagnetic medium. The essence of the method consists of rapid levitational assembly in the construct's heterogenous magnetic field from various materials, such as, for example, tissue spheroids, single-cell suspension, microorganisms, peptides, potassium phosphate granules, which are chaotically distributed in the volume of culture medium. The construct is formed in a specific area where a magnetic trap is formed as a result of the combined forces of gravitational and magnetic fields. In this area, the gravitational pull is compensated and particles of material are forced together. The introduced technology can become a powerful biofabrication tool that enables rapid assembly of various three-dimensional constructs, including biological tissues and organs.