METHOD FOR QUANTIFYING 3,6-ANHYDRO-L-GALACTOSE USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND DIFFERENTIAL REFRACTOMETRIC DETECTOR

METHOD FOR QUANTIFYING 3,6-ANHYDRO-L-GALACTOSE USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND DIFFERENTIAL REFRACTOMETRIC DETECTOR

The present invention relates to a method for quantifying 3,6-anhydro-L-galactose using high-performance liquid chromatography and a differential refractometric index detector, whereby L-AHG and D-galactose, which are saccharification products of agarose or agar, can be independently quantified by c...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: KIM, Kyoung Heon, CHO, Kyung-mun, KIM, Dong-Hyun, YUN, Eun-Ju
Format: Patent
Sprache:eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:The present invention relates to a method for quantifying 3,6-anhydro-L-galactose using high-performance liquid chromatography and a differential refractometric index detector, whereby L-AHG and D-galactose, which are saccharification products of agarose or agar, can be independently quantified by controlling the separation conditions of the high-performance liquid chromatography/differential refractive index detector, even though the two materials are difficult to separate due to having similar molecular weights and structures.