ACOUSTIC ENERGY BASED CELL LYSIS AND NUCLEIC ACID FRAGMENTATION

ACOUSTIC ENERGY BASED CELL LYSIS AND NUCLEIC ACID FRAGMENTATION

A method for lysing cells and shearing genomic DNA to reduce viscosity of the cell lysate. Cells may be lysed to release cell lysate, and the cell lysate may be treated with focused acoustic energy to shear genomic DNA so that the genomic DNA is sheared to DNA fragments having a fragment size no lar...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: LAUGHARN, JR., James A, Thomann, Hans-Ulrich
Format: Patent
Sprache:eng
Schlagworte:
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A method for lysing cells and shearing genomic DNA to reduce viscosity of the cell lysate. Cells may be lysed to release cell lysate, and the cell lysate may be treated with focused acoustic energy to shear genomic DNA so that the genomic DNA is sheared to DNA fragments having a fragment size no larger than 50% of the starting base pair size of the genomic DNA. Lysing and DNA shearing may be done by acoustic energy and in a single vessel, allowing for automated handling of cell lysate.