IMPROVED METHODS OF ANTIBODY PRODUCTION THAT MINIMIZE DISULFIDE BOND REDUCTION

IMPROVED METHODS OF ANTIBODY PRODUCTION THAT MINIMIZE DISULFIDE BOND REDUCTION

The present disclosure discloses methods for purifying antibodies providing an air overlay or headspace to single-use storage bags containing the antibody during purification. In specific embodiments, the application relates to purification of antibodies to human TIGIT (T cell immunoreceptor with Ig...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: O'MARA, Brian W, FANN, John C, SMITH, Laura R
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
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Beschreibung
Zusammenfassung:The present disclosure discloses methods for purifying antibodies providing an air overlay or headspace to single-use storage bags containing the antibody during purification. In specific embodiments, the application relates to purification of antibodies to human TIGIT (T cell immunoreceptor with Ig and ITIM domains), such as antibody 22G2, which decrease unwanted disulfide bond reduction. The application further provides depth filtration at lower throughput and/or flux, which also minimizes unwanted disulfide bond reduction.