Computer Files and Methods Supporting Forensic Analysis of Nucleotide Sequence Data

In one illustrative embodiment, an allelotyping method may comprise using a massively parallel sequencing (MPS) instrument to read nucleotide sequences in a sample and to generate nucleotide sequence data quantifying each read of a nucleotide sequence in the sample, determining, for each read by the MPS instrument, whether a portion of the generated nucleotide sequence data represents a short tandem repeat (STR) associated with a corresponding locus, adding each portion of the nucleotide sequence data determined to represent an STR to a locus-specific list for the corresponding locus, determining, for each locus-specific list, a number of occurrences within that locus-specific list of identical nucleotide sequence data representing a unique STR, and identifying each unique STR for which the number of occurrences of identical nucleotide sequence data within the locus-specific list exceeds an abundance threshold as an allele of the corresponding locus for the sample.