SYSTEM AND METHOD FOR REAL-TIME PRIORITIZATION OF SEQUENCING
A method (100) for prioritizing sequencing of nucleic acid molecules from two or more sources, comprising: (i) ligating (120) one or more ends of each of a plurality of nucleic acid molecules to an adapter, wherein the adaptor is unique to each source and comprises a unique identifier nucleic acid s...
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Format: | Patent |
Sprache: | eng |
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Zusammenfassung: | A method (100) for prioritizing sequencing of nucleic acid molecules from two or more sources, comprising: (i) ligating (120) one or more ends of each of a plurality of nucleic acid molecules to an adapter, wherein the adaptor is unique to each source and comprises a unique identifier nucleic acid sequence; (ii) pooling (130) the nucleic acid molecules from each of the two or more sources; (iii) sequencing (140) a plurality of nucleotides of an identifier nucleic acid sequence of an adapter; (iv) determining (150) in real-time from which of the two or more sources the nucleic acid molecule being sequenced came; (v) determining (160), from a prioritization rule set and based on the determined source, a sequencing priority for the nucleic acid molecule; and (vi) allowing (170) the sequencing process to proceed, or modifying (180) the sequencing process, based on the determined sequencing priority. |
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