Polypeptide nano-bubbles and preparation method and application thereof

A preparation method for polypeptide nano-bubbles, comprising the following steps: constructing a recombinant plasmid, which includes a Flag tag, an adipose tissue-targeting polypeptide and the coding gene of nano-bubble marker membrane protein CD63; and transfecting the recombinant plasmid into cel...

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Bibliographische Detailangaben
Hauptverfasser:	WU, Heming, TANG, Ningyuan, LI, Hongwei, WANG, Jingjing, YU, Tingting, ZHANG, Yaqin, QIAN, Bin, HAN, Xiao, XU, Rufeng, YUAN, Yi, CHANG, Xiaoai, WU, Jindao, PANG, Jing, PU, Liyong
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMISTRY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
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Beschreibung
Zusammenfassung:	A preparation method for polypeptide nano-bubbles, comprising the following steps: constructing a recombinant plasmid, which includes a Flag tag, an adipose tissue-targeting polypeptide and the coding gene of nano-bubble marker membrane protein CD63; and transfecting the recombinant plasmid into cells which secrete nano-bubbles through lipidosome for culturing, collecting a cell culture solution, and extracting the polypeptide nano-bubbles by ultracentrifugation. The present invention further discloses the polypeptide nano-bubbles and application thereof in the preparation of drugs for treating obesity. The polypeptide nano-bubbles bring great convenience for targeted therapy of anti-obesity drugs. The polypeptide nano-bubbles have good biocompatibility and are capable of carrying different kinds of bioactive substances.