COMPETITIVE SMALL MOLECULE DETECTION ASSAYS USING ARRAYED IMAGING REFLECTOMETRY

COMPETITIVE SMALL MOLECULE DETECTION ASSAYS USING ARRAYED IMAGING REFLECTOMETRY

Understanding the amount of exposure individuals have had to common chemical pollutants critically requires the ability to detect those compounds in a simple, sensitive, and specific manner. Doing so using label-free biosensor technology has proven challenging, however, given the small molecular wei...

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Bibliographische Detailangaben
Hauptverfasser: CARTER, Jared A, STREIMER, Christopher C, TRIPLETT, Emily J, MILLER, Benjamin L
Format: Patent
Sprache:eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Zusammenfassung:Understanding the amount of exposure individuals have had to common chemical pollutants critically requires the ability to detect those compounds in a simple, sensitive, and specific manner. Doing so using label-free biosensor technology has proven challenging, however, given the small molecular weight of many pollutants of interest. To address this issue, a pollutant microarray based on the label-free Arrayed Imaging Reflectometry (AIR) detection platform was developed. The sensor that has undergone a two-step blocking process is able to detect three common environmental contaminants (benzo[a]pyrene 200, bisphenol A, and acrolein) in human serum via a competitive binding scheme.