CELLULOLYTIC COMPOSITIONS COMPRISING MONOOXYGENASE POLYSACCHARIDE ENZYMES WITH IMPROVED ACTIVITY

CELLULOLYTIC COMPOSITIONS COMPRISING MONOOXYGENASE POLYSACCHARIDE ENZYMES WITH IMPROVED ACTIVITY

The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This...

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Bibliographische Detailangaben
Hauptverfasser: RAMOS MARTÍN, Juan Luis, REYES SOSA, Francisco Manuel, LEDESMA GARCÍA, Laura, DÍEZ GARCÍA, Bruno, ROCHA MARTÍN, Javier, PLATERO GÓMEZ, Ana Isabel, MARTÍN PÉREZ, Lucía, ÁLVAREZ NÚÑEZ, Consolación, MORENO PÉREZ, Antonio Javier, PÉREZ GÓMEZ, Dolores, VALBUENA CRESPO, Noelia, VIÑAS DE LA CRUZ, Laura, BERMÚDEZ ALCÁNTARA, María de los Angeles, SÁNCHEZ ZAMORANO, Laura, GAVALDÁ MARTÍN, Sandra
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.