AFFINITY CHROMATOGRAPHY PURIFICATION WITH LOW CONDUCTIVITY WASH BUFFER

AFFINITY CHROMATOGRAPHY PURIFICATION WITH LOW CONDUCTIVITY WASH BUFFER

Herein is reported a method for producing a bispecific antibody comprising a first antigen-binding site that specifically binds to a first antigen and a second antigen-binding site that specifically binds to a second antigen comprising the following stepsa) cultivating a cell comprising a nucleic ac...

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Bibliographische Detailangaben
Hauptverfasser: FALKENSTEIN, Roberto, WILLMANN, Steffen, ROSENBERG, Eva, BIALAS, Agathe
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:Herein is reported a method for producing a bispecific antibody comprising a first antigen-binding site that specifically binds to a first antigen and a second antigen-binding site that specifically binds to a second antigen comprising the following stepsa) cultivating a cell comprising a nucleic acid encoding the bispecific antibody,b) recovering the bispecific antibody from the cell or the cultivation medium,c) contacting the bispecific antibody with an affinity chromatography material,d) washing the affinity chromatography material with a low conductivity aqueous solution, wherein the low conductivity aqueous solution has a conductivity value of about 0.5 mS/cm or less,e) recovering the bispecific antibody from the affinity chromatography material,f) performing a further chromatography stepand thereby producing the bispecific antibody.