NUCLEIC ACID AMPLIFIER, CARTRIDGE FOR NUCLEIC ACID AMPLIFICATION AND NUCLEIC ACID AMPLIFICATION METHOD

A nucleic amplifier is provided to reduce an amplification efficiency drop even with a short cycle time. In the nucleic amplifier, the denaturation phase of moving and retaining a liquid droplet in a first region of a container heated to a denaturation temperature of a target nucleic acid, and the s...

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Bibliographische Detailangaben
1. Verfasser:	UEHARA Masayuki
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A nucleic amplifier is provided to reduce an amplification efficiency drop even with a short cycle time. In the nucleic amplifier, the denaturation phase of moving and retaining a liquid droplet in a first region of a container heated to a denaturation temperature of a target nucleic acid, and the synthesis phase of moving and retaining the liquid droplet in a second region of the container different from the first region are repeated in multiple cycles. The liquid droplet contains a fluorescently labeled probe. The fluorescently labeled probe contains a minor groove binder molecule.