METHODS FOR SELECTING ENZYMES HAVING ENHANCED ACTIVITY

Provided herein are methods and means for enhancing enzymatic activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a gene and a marked enzymatic substrate both directly linked to a solid phase. Expressed enzymes with greater a...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	Emrich Charles, Svendsen Allan, Toriello Nicholas, Blazej Robert
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Provided herein are methods and means for enhancing enzymatic activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a gene and a marked enzymatic substrate both directly linked to a solid phase. Expressed enzymes with greater activity will preferentially release the selectable marker from the solid phase, whereas enzymes with less activity will leave the markers intact. Removal of the marked compounds provides an enriched gene library encoding for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods.