SYSTEM AND METHOD FOR MELTING CURVE NORMALIZATION

The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems...

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Bibliographische Detailangaben
Hauptverfasser: Pearson Ken, Paquerault Sophie, Huang Hung, Paek Jeanette, Yang Yang, Denney Bradley Scott, Charlton Lance, Uppal Tejinder, Seikh Attaullah, Parthasarathy Vyshnnavi
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).