CYTOMETRIC MECHANISM, CELL CULTURE DEVICE COMPRISING SAME, AND CYTOMETRIC METHOD

A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow ce...

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Bibliographische Detailangaben
Hauptverfasser: KAWARAI Masako, SAKURAI Toshinari, SHIMASE Akihiro, KOSHI Hiroyuki
Format: Patent
Sprache:eng
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Zusammenfassung:A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow cell, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.