EXPERIMENTAL METHODS FOR CONDUCTING COMPETITIVE BINDING ASSAYS

EXPERIMENTAL METHODS FOR CONDUCTING COMPETITIVE BINDING ASSAYS

The present disclosure is directed toward improved methods of conducting a competitive binding assay or experiment. The methodology includes utilizing either a positive control, a negative control, or both in order to scale experimentation results to results that can be utilized to obtain a precise...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: GLASS THOMAS R, LACKIE STEVE J
Format: Patent
Sprache:eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:The present disclosure is directed toward improved methods of conducting a competitive binding assay or experiment. The methodology includes utilizing either a positive control, a negative control, or both in order to scale experimentation results to results that can be utilized to obtain a precise measurement of the kinetic rate constant (the off rate denoted as koff) describing the dissociation of a non-covalent complex such as an antibody antigen complex, receptor ligand complex, etc.