Electrospun Nanofibrous Membranes and Disposable Glucose Biosensor

An electrospun nanofibrous membrane being sheet like and being formed by comprising multiple glucose oxidase/potassium hexacyanoferrate (III) modified electrospun nanofibers, wherein the glucose oxidase/potassium hexacyanoferrate (III) modified electrospun nanofibers are PVA electrospun nanofibers c...

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Bibliographische Detailangaben
Hauptverfasser:	YU HSIU-AN, WU CHANG-MOU, HUANG CHUNUNG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY PERFORMING OPERATIONS PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	An electrospun nanofibrous membrane being sheet like and being formed by comprising multiple glucose oxidase/potassium hexacyanoferrate (III) modified electrospun nanofibers, wherein the glucose oxidase/potassium hexacyanoferrate (III) modified electrospun nanofibers are PVA electrospun nanofibers containing glucose oxidase and potassium hexacyanoferrate (III) homogeneously dispersed therein; and the glucose oxidase/potassium hexacyanoferrate (III) modified electrospun nanofibers are PVA electrospun nanofibers and are cross-linked by glutaraldehyde vapor with ultrasonic energy assistance. In the present invention, graphene modified PVA/GOx electrospun membranes were prepared to examine the immobilization mechanism between graphene and GOx. The electrochemical measurement results show that the sensitivities increased with increasing graphene concentrations up to 20 ppm. The highest sensitivity recorded 38.7 μA/mM was for a PVA/GOx membrane with 20 ppm graphene representing a 109% increase over a membrane made without graphene.