METHODS OF SITE-DIRECTED TRANSFORMATION

METHODS OF SITE-DIRECTED TRANSFORMATION

Methods of integrating exogenous DNA into the genome of a eucaryotic organism comprising at least one recombination site are effected by contacting the genome with a DNA molecule comprising selected DNA and at least one site-specific recombination site which is compatible with a site-specific recomb...

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1. Verfasser: LOWE BRENDA A
Format: Patent
Sprache:eng
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Methods of integrating exogenous DNA into the genome of a eucaryotic organism comprising at least one recombination site are effected by contacting the genome with a DNA molecule comprising selected DNA and at least one site-specific recombination site which is compatible with a site-specific recombination site in the genome. DNA insertion is catalyzed by the presence of a recombinase effective for the compatible recombination sites. Selected DNA can be integrated into a genome without removal of DNA from said organism. The methods are useful for site-specific insertion of selected DNA into plants to provide fertile transgenic plants and progeny seed.