DETERMINATION METHOD, DETERMINATION DEVICE, DETERMINATION SYSTEM, AND COMPUTER PROGRAM

A determination method for determining reliability of a selective binding amount of a substance to be examined obtained as detection intensity of a label when a labeled substance to be examined binds to a selective binding substance fixed as a spot on a carrier includes: determining a position of the spot in image data obtained by imaging the detection intensity in the carrier and extracting a pixel group corresponding to the spot; calculating a ratio or a difference between a median value of the detection intensity of the pixel group extracted at the determining and a median value of the detection intensity of the pixel group excluding a certain top proportion of and/or a certain bottom proportion of pixels; and determining quality of the reliability based on the ratio or the difference calculated at the calculating and a certain reference value.