METHOD FOR QUANTITATIVELY EVALUATING ANTIMICROBIAL ACTIVITY OF ANTIMICROBIAL SAMPLE USING DISC DIFFUSION METHOD AND SYSTEM FOR MEASURING ANTIMICROBIAL ACTIVITY USING THE SAME
A method for quantitatively evaluating the antimicrobial activity of an antimicrobial sample using a disc diffusion method, and a measurement system using the method are provided. The method comprises: (a) measuring a maximum inhibition zone width (IZWmax) for a first sample, in which a colony is ex...
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Zusammenfassung: | A method for quantitatively evaluating the antimicrobial activity of an antimicrobial sample using a disc diffusion method, and a measurement system using the method are provided. The method comprises: (a) measuring a maximum inhibition zone width (IZWmax) for a first sample, in which a colony is expected not to be observed, using a disc diffusion method, and measuring a colony forming unit (CFU0) value for the first sample using a colony counting method; (b) measuring a microbial growth inhibition zone width (IZWi) and a colony forming unit (CFUi) value for a second sample having any antimicrobial activity; (c) calculating α value and value based on the results of steps (a) and (b) using equations (10) and (20); (d) measuring a microbial growth inhibition zone width (IZWx) for a measurement sample; and (e) calculating a quantitative antimicrobial activity value ( ) using equation (30). |
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