Expression Cassette, Use of the Expression Cassette, Expression Vector, Prokaryotic Host Cell Comprising Expression Vector, Bacterial Strain and a Method for Producing a Polypeptide

The subject matters of invention relate to expression cassette, use of the expression cassette, expression vector, prokaryotic host cell harbouring expression vector, bacterial strain, and method for producing a polypeptide. In more detail, the invention relate to stable expression of recombinant po...

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Hauptverfasser: KESIK-BRODACKA MALGORZATA, PLUCIENNICZAK GRAZYNA, PLUCIENNICZAK ANDRZEJ, ROMANIK AGNIESZKA, MIKIEWICZ-SYGULA DIANA
Format: Patent
Sprache:eng
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Zusammenfassung:The subject matters of invention relate to expression cassette, use of the expression cassette, expression vector, prokaryotic host cell harbouring expression vector, bacterial strain, and method for producing a polypeptide. In more detail, the invention relate to stable expression of recombinant polypeptides, in systems with no need of using antibiotics. Particularly, stable expression of recombinant proteins obtained with the use of very efficient T7 polymerase/promoter system.