AUTOMATED IMMUNOANALYZER SYSTEM FOR PERFORMING DIAGNOSTIC ASSAYS FOR ALLERGIES AND AUTOIMMUNE DISEASES

A quantitative method for performing an automated diagnostic assay, comprising: incubating a capture reagent with a streptavidin-coated medium to form a solid phase complex; washing the solid phase complex to remove excess capture reagent; incubating the solid phase complex with a serum sample to fo...

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Hauptverfasser: WESTON JAMES, SARGEANT BRUCE ALAN, KNOX TRAVIS, CUARESMA JACALNE FRAN ZYLO, MCMENAMY EVAN PHILLIP, TAYLOR TERI, HUNG VICTORIA, TRONDLE LINDA MARIE, MOSKALEV ANATOLY, NIE YILING, CANFIELD DOUGLAS JOHN, REID TAYLOR ADDISON, HWANG TAE HO, DIAMOND RONALD NORMAN, TUVIVO STEPHANIE, STACK MARINELA GOMBOSEV, RELIFORD MORKOAH BLAY, BAO-GUEY CHAN JENNIFER, SINSON EDSEL LAWRENCE NOCHE, RIEGER DENNIS EDWIN, SCHELL RACHEL SARAH, VANDE WETERING SCOTT WILLIAM, GANN STEVE MICHAEL, HALL ERIC DARNELL, LUO YI, MORTON JOHN LEWIS, VAN CLEVE MARK DAVID, RAGHAVAN NANDITHA
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Sprache:eng
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Zusammenfassung:A quantitative method for performing an automated diagnostic assay, comprising: incubating a capture reagent with a streptavidin-coated medium to form a solid phase complex; washing the solid phase complex to remove excess capture reagent; incubating the solid phase complex with a serum sample to form an immune complex; washing the immune complex to remove any unbound sample; incubating the immune complex with a conjugate to create an immune-conjugate complex; washing the immune-conjugate complex to remove any unbound conjugate; introducing a substrate capable of generating a quantifiable response; and calibrating the response generated from introducing the substrate.