MULTICOPPER OXIDASE MUTANT, A GENE CODING THEREOF, AND A BIOFUEL-CELL USING THE SAME

A multicopper oxidase mutant having improved resistance to imidazole compounds is provided. A multicopper oxidase mutant, which comprises an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of either or both an amino acid residue corresponding to methion...

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Bibliographische Detailangaben
Hauptverfasser:	KUSUMEGI TAKAHIRO, TERADA KUMI
Format:	Patent
Sprache:	eng
Schlagworte:	BASIC ELECTRIC ELEMENTS BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ELECTRICITY ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSIONOF CHEMICAL INTO ELECTRICAL ENERGY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A multicopper oxidase mutant having improved resistance to imidazole compounds is provided. A multicopper oxidase mutant, which comprises an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of either or both an amino acid residue corresponding to methionine at position 157 and an amino acid residue corresponding to proline at position 414 with a different amino acid and has activity of catalyzing a reaction generating water molecules via four-electron reduction of oxygen molecules using ABTS (2,2′-azinobis(3-ethylbenzoline-6-sulfonate)) as a substrate is provided.